The RealBest diagnostic kits are intended for the detection of infectious agents and for the determination of genetic polymorphisms by real-time PCR. In-house developed technologies turn the procedure of real-time PCR analysis from a complicated process, requiring extensive specialized knowledge and practical experience, into a standardized, simple and readily reproducible procedure. AO Vector-Best provides convenient techniques in diagnostics such as lyophilized, ready-to-use mixtures with a long shelf-life and the possibility to be transported at room temperature.
Str-format: Ready Master Mix is lyophilized in 0.2 ml white tubes of the 96-tube plate. Simple addition of extracted DNA/RNA samples is required for the reaction procedure.
Fla-format: Master Mix for 10 reactions is lyophilized in 2.0 ml vials. After dilution with Recovery Solution, dispense the Master Mix to the optimized tubes, add extracted DNA/RNA and run the test.
Uni-format: Ready Master Mix is lyophilized in clear 0.2 ml tubes. Add extracted DNA/RNA into the tubes and run the test. One tube – one reaction.
Str, Uni and Fla formats can be used with: CFX96™ (Bio-Rad, USA), DTprime (DT-96), DTlite (DNA-Technology, Russia).
Fla, Uni can be used with: Rotor-Gene® Q (Qiagen, Germany), Rotor-Gene® 6000/3000 (Corbett Research, Australia).
It is possible to use other thermal cyclers with similar technical characteristics, but their application requires additional validation.