Accurate diagnostics – effective treatment! EN RU

The RealBest diagnostic kits are intended for the detection of infectious agents and for the determination of genetic polymorphisms by real-time PCR. In-house developed technologies turn the procedure of real-time PCR analysis from a complicated process, requiring extensive specialized knowledge and practical experience, into a standardized, simple and readily reproducible procedure. AO Vector-Best provides convenient techniques in diagnostics such as lyophilized, ready-to-use mixtures with a long shelf-life and the possibility to be transported at room temperature.

RealBest kits offer:
  • Complex approach to PCR diagnostics encompassing sample preparation, PCR and evaluation of results
  • Simple nucleic acids extraction using magnetic particles
  • Lyophilized Ready Master Mix for the detection of DNA/RNA in real-time PCR
  • Universal cycler protocol for the detection of a wide range of pathogens
  • Noncompetitive Internal Control
  • Storage of all components at the same temperature (2-8)°С for up to 12 months; no freezing required
  • Transportation of kits at room temperature for up to 10 days
  • The majority of “RealBest” kits are CE-marked (Declarations of conformity of 2017/12/21, of 2018/08/29, of 2018/09/26, of 2019/03/26, CE0483 № D1213100015)
Different formats:

Str-format: Ready Master Mix is lyophilized in 0.2 ml white tubes of the 96-tube plate. Simple addition of extracted DNA/RNA samples is required for the reaction procedure.

Fla-format: Master Mix for 10 reactions is lyophilized in 2.0 ml vials. After dilution with Recovery Solution, dispense the Master Mix to the optimized tubes, add extracted DNA/RNA and run the test.

Uni-format: Ready Master Mix is lyophilized in clear 0.2 ml tubes. Add extracted DNA/RNA into the tubes and run the test. One tube – one reaction.

 

Real-time PCR cyclers and formats:

Str, Uni and Fla formats can be used with: CFX96™ (Bio-Rad, USA), DTprime (DT-96), DTlite (DNA-Technology, Russia).

Fla, Uni can be used with: Rotor-Gene® Q (Qiagen, Germany), Rotor-Gene® 6000/3000 (Corbett Research, Australia).

It is possible to use other thermal cyclers with similar technical characteristics, but their application requires additional validation.

Diagnostic kits